玉米miR167-ARF3/30-PAO1模块通过调控H2O2产生介导对玉米褪绿斑驳病毒的抗性
发布日期：2022-03-20
Abstract:Maize chlorotic mottle virus (MCMV) is the key pathogen causing maize lethal necrosis (MLN). Due to the sharply increased incidence of MLN in many countries, there is an urgent need to identify resistant lines and uncover the underlying resistance mechanism. Here, we showed that the abundance of maize (Zea mays) microR167 (Zma-miR167) positively modulates the degree of resistance to MCMV. Zma-miR167 directly targets Auxin Response Factor3 (ZmARF3) and ZmARF30, both of which negatively regulate resistance to MCMV. RNA-sequencing coupled with gene expression assays revealed that both ZmARF3 and ZmARF30 directly bind the promoter of Polyamine Oxidase 1 (ZmPAO1) and activate its expression. Knockdown or inhibition of enzymatic activity of ZmPAO1 suppressed MCMV infection. Nevertheless, MCMV-encoded p31 protein directly targets ZmPAO1 and enhances the enzyme activity to counteract Zma-miR167-mediated defense to some degree. We uncovered a role of the Zma-miR167-ZmARF3/30 module for restricting MCMV infection by regulating ZmPAO1 expression, while MCMV employs p31 to counteract this defense.
论文链接：Plant Physiology在线 https://doi.org/10.1093/plphys/kiac099
玉米褪绿斑驳病毒（maize chlorotic mottle virus, MCMV）是玉米上最具破坏性的病原之一，其通常与马铃薯Y病毒科（Potyviridae）的一种成员复合侵染引起玉米致死性坏死病（Maize Lethal Necrosis, MLN），造成严重的产量损失，甚至绝收。该研究在玉米上鉴定到首个抗MCMV的microRNA（Zma-miR167），并揭示了玉米miR167-ARF3/30-PAO1信号通路发挥抗MCMV功能，为培育抗MCMV玉米材料提供了重要参考。
