Single-cell RNA sequencing profiles reveal cell type-specific transcriptional regulation networks conditioning fungal invasion in maize roots
发布日期：2023-06-25
Abstract：Stalk rot caused by Fusarium verticillioides (Fv) is one of the most destructive diseases in maize production. The defence response of root system to Fv invasion is important for plant growth and development. Dissection of root cell type-specific response to Fvinfection and its underlying transcription regulatory networks will aid in understanding the defence mechanism of maize roots to Fv invasion. Here, we reported the transcriptomes of 29 217 single cells derived from root tips of two maize inbred lines inoculated with Fv and mock condition, and identified seven major cell types with 21 transcriptionally distinct cell clusters. Through the weighted gene co-expression network analysis, we identified 12 Fv-responsive regulatory modules from 4049 differentially expressed genes (DEGs) that were activated or repressed by Fv infection in these seven cell types. Using a machining-learning approach, we constructed six cell type-specific immune regulatory networks by integrating Fv-induced DEGs from the cell type-specific transcriptomes, 16 known maize disease-resistant genes, five experimentally validated genes (ZmWOX5b, ZmPIN1a, ZmPAL6, ZmCCoAOMT2, and ZmCOMT), and 42 QTL or QTN predicted genes that are associated with Fv resistance. Taken together, this study provides not only a global view of maize cell fate determination during root development but also insights into the immune regulatory networks in major cell types of maize root tips at single-cell resolution, thus laying the foundation for dissecting molecular mechanisms underlying disease resistance in maize.
茎腐病是玉米最重要的全球性病害之一，引起玉米产量损失，病害导致的倒伏是玉米机收的制约因素，病原菌产生的毒素影响食品和饲料安全。玉米茎腐病致病菌复杂多变，且在玉米整个生育期对多个部位进行侵染，使得病害抗性机理研究尤为复杂。在我国玉米主产区，拟轮枝镰孢菌（Fusarium verticillioides, Fv）已成为茎腐病的重要致病菌，也是玉米穗腐病的主要致病因子。然而，有关玉米对Fv引起的镰孢茎腐病（FSR）的抗性研究甚少，在Fv与玉米植株之间的互作及玉米抵御Fv入侵的分子机制和调控网络方面均不明确。在当前小麦玉米连作、玉米大豆复合种植等日趋成为主流耕作制度及机械收获、秸秆还田等措施广泛应用的背景下，开展FSR抗性分子机理和调控途径研究，对玉米抗病育种具有重要意义。
在Plant Biotechnology Journal在线发表题为“Single-cell RNA sequencing profiles reveal cell type-specific transcriptional regulation networks conditioning fungal invasion in maize roots”研究论文，通过单细胞测序绘制玉米根尖高分辨率细胞图谱，阐明玉米根尖主要细胞类型对镰孢菌侵染根系的应答机制和关键基因调控模块，结合整合生物信息学及机器学习等方法首次构建了玉米根尖免疫调控网络，加深了对玉米精准调控根尖生长发育与抵御真菌侵染平衡过程的认识，为解析玉米抗病分子机制及抗病育种奠定了基础。
Source：https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.14097
