Involvement of a Polyketide Synthetase CIPKS18 in the Regulation of Vegetative Growth, Melanin and Toxin Synthesis, and Virulence in Curvularia lunata

发布日期：2021-01-31

Author:Gao Jin-Xin; Chen Jie*

Abstract:

Theclpks18gene was first cloned and identified inCurvularia lunata.It contains 6571 base pairs (bp) and an 6276 bp open reading frame encoding 2091 amino acids. The ClPKS18 deletion mutant displayed an albino phenotype, and almost lost the ability to product 5-(hydroxymethyl) furan-2-carboxylate (M5HF2C) toxin, implying thatclpks18gene inC. lunatais not only involved in 1,8-dihydroxynaphthalene melanin synthesis, but also relatively associated with M5HF2C toxin biosynthesis of the pathogen. The pathogenicity assays revealed that ΔClPKS18 was impaired in colonizing the maize leaves, which corresponds to the finding that ClPKS18 controls the production of melanin and M5HF2C inC. lunata. Results indicate that ClPKS18 plays a vital role in regulating pathogenicity of inC. lunata.

Key Words:melanin PKS18 toxin

Source:Plant Pathology Journal 2017,33(6):597-601